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The method contains all the GC)Tj (-)Tj (MS parameters needed for the analysis.)Tj ( )Tj 1.5 -1.66 Td (1.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (If you are not in the \223Instrumen)Tj (t Control\224 window, open it by choosing \223Instrument )Tj 0 -1.18 TD (Control\224 from the View menu.)Tj ( )Tj 0 -1.16 TD (Click on the Method menu, and select Load\205)Tj ( )Tj -1.5 -1.16 Td (2.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Select the method \223org1.m\224.)Tj ( )Tj -1.5 -1.18 Td (3.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (After the method has been loaded, click on the button with the large gre\ en arrow to )Tj T* (start the analysis.)Tj ( )Tj -1.5 -1.16 Td (4.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (E)Tj (nter your name in the \223Operator Name\224 box that appears)Tj ( )Tj -1.5 -1.18 Td (5.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Enter \223org1.d\224 in the data file name box. Note: normally we do not\ wish to save )Tj T* (these chromatograms, so we will continuously overwrite to this file!)Tj 25.942 0 Td ( )Tj -27.442 -1.16 Td (6.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Enter the name of your sample in the \223Sample Name\224 b)Tj (ox.)Tj ( )Tj -1.5 -1.18 Td (7.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (If you desire, enter any miscellaneous information in the \223Misc. 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N)Tj (ote: )Tj -22.753 -1.18 Td (a Pasteur pipet contains about 2 mL of fluid when filled.)Tj ( )Tj -1.5 -1.16 Td (13.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Using another glass Pasteur pipet, add 1)Tj 6.96 0 0 6.96 311.6848 343.92 Tm ( )Tj 12 0 0 12 313.4348 344.88 Tm (\226)Tj 6.96 0 0 6.96 319.4348 343.92 Tm ( )Tj 12 0 0 12 321.1848 344.88 Tm (2 drops of your unknown sample to the )Tj -16.265 -1.16 Td (CH)Tj 6.96 0 0 6.96 144.0237 330 Tm (2)Tj 12 0 0 12 147.5237 330.96 Tm (Cl)Tj 6.96 0 0 6.96 158.8393 330 Tm (2)Tj 12 0 0 12 162.3394 330.96 Tm (, and mix.)Tj ( )Tj -4.528 -1.18 Td (14.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Take a 10 \265L GC)Tj (-)Tj (MS syringe $make sure it has a blunt, cone shaped tip$ and pull )Tj T* (the plunger bac)Tj (k to the 1 \265L mark. Place the tip of the needle in your sample, and )Tj T* (withdraw the plunger to the 2 \265L mark. Remove the tip of the needle f\ rom your )Tj 0 -1.18 TD (sample, and pull back the plunger to the 3 \265L mark.)Tj ( )Tj -1.5 -1.16 Td (15.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Your unknown sample is now held between two air gaps.)Tj ( )Tj -1.5 -1.16 Td (16.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Hold)Tj ( )Tj (the plunger in place while you align the syringe over the injection port\ . We are )Tj T* (using injection port B, which is the port the furthest from the front of\ the GC. Inject )Tj 0 -1.16 TD (the sample by inserting the syringe needle into the injector until the b\ arrel of the )Tj T* (s)Tj (yringe rests on the injector. Press the plunger all the way into the syr\ inge. 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Otherwise, wait for\ the data to be )Tj T* (collected, and choose \223Load Data File\224 from the \223Fi)Tj (le\224 menu. Your data file name )Tj T* (should be \223Org1.d\224.)Tj ( )Tj -1.5 -1.2 Td (19.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Make sure the Org1.m method has been loaded. It should say \223ORG1.M\224\ in the )Tj T* (title bar at the top of the screen. If it does not, then click on the \223\ Method\224 menu, )Tj T* (and select \223Load Method\205\224. 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You )Tj T* (may also average several data points together by drawing a rectangle ove\ r the )Tj T* (chromatogram peaks with t)Tj (he )Tj /T1_1 1 Tf (right)Tj /T1_0 1 Tf ( )Tj (mouse button depressed.)Tj ( )Tj -1.5 -1.2 Td (23.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (If you double right click on the mass spectrum, this will search the NBS\ 75K library )Tj T* (of 75,000 organic compounds.)Tj ( )Tj -1.5 -1.2 Td (24.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (You may print a copy of the mass spectrum and the chromatogram by select\ ing )Tj T* (\223Print\224 from the \223File\224 menu. If y)Tj (ou select \223TIC and Spectrum\224, you will receive a )Tj T* (print)Tj (-)Tj (out of the TIC $total)Tj (-)Tj (ion)Tj (-)Tj (chromatogram$ and the mass spectrum you selected.)Tj ( )Tj -1.5 -1.2 Td (25.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (If you need to integrate your chromatogram, select \223Autointegrate\224\ from the )Tj T* (\223Chromatogram\224 menu. Do not save the autointegrat)Tj 20.8 0 Td (ion parameters to the Method. )Tj -20.8 -1.2 Td (You can view and print the integration report by selecting \223Integrati\ on Results\205\224 or )Tj T* (\223Percent Report\224 from the same menu.)Tj ( )Tj -1.5 -1.2 Td (26.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (When you are finished analyzing your chromatogram, you can quit the )Tj /T1_1 1 Tf 27.668 0 Td (Data )Tj -27.668 -1.2 Td (Analysis)Tj /T1_0 1 Tf ( )Tj (program by selecting \223)Tj (Exit\224 from the \223File\224 menu.)Tj ( )Tj /TT0 1 Tf 0.003 Tc -0.002 Tw 13.92 0 0 13.92 90 285.6 Tm [(F)1(i)1(ni)1(s)1(hi)1(ng)1( Up)]TJ 0 Tc 0 Tw 4.857 0 Td ( )Tj /T1_0 1 Tf 12 0 0 12 108 267.6 Tm (27.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (If you are finished with your unknown sample, throw the solvent away in \ the )Tj T* (Halogenated Organic Solvent waste bottle located in the Northern most fu\ me hood )Tj T* (in the Organic Chemistry lab.)Tj ( )Tj -1.5 -1.2 Td (28.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (It is very important to clean)Tj ( )Tj (the syringe out after every injection. You can do this by )Tj T* (putting a small amount of acetone in a beaker, and drawing up 5)Tj 6.96 0 0 6.96 426.2341 209.04 Tm ( )Tj 12 0 0 12 427.9841 210 Tm (\226)Tj 6.96 0 0 6.96 433.9841 209.04 Tm ( )Tj 12 0 0 12 435.7341 210 Tm (10 \265L of acetone )Tj -25.811 -1.2 Td (into the syringe, followed by squirting it out into a kim)Tj 21.349 0 Td (-)Tj (wipe $where it will quickly )Tj -21.349 -1.2 Td (evaporate$. You should do th)Tj (is 5)Tj 6.96 0 0 6.96 276.5371 180.24 Tm ( )Tj 12 0 0 12 278.2871 181.2 Tm (\226)Tj 6.96 0 0 6.96 284.2871 180.24 Tm ( )Tj 12 0 0 12 286.0371 181.2 Tm (6 times to ensure the syringe is clean. Failure to do )Tj -13.336 -1.2 Td (this will result in carry over of your sample into the next sample intro\ duced into the )Tj T* (syringe. Also, the plunger may freeze)Tj (-)Tj (up if the sample is not washed off of the inside )Tj T* (surfaces of the syrin)Tj (ge.)Tj ( )Tj ET endstream endobj 3 0 obj <>stream 2013-01-11T21:02:59Z Word 2013-01-11T16:03:05-05:00 2013-01-11T16:03:05-05:00 Adobe Mac PDF Plug-in application/pdf Andrew M. Napper Microsoft Word - GCMS of generic organics.doc uuid:324a046c-d9fa-ff49-8cfb-675b042dd356 uuid:dc4bc15c-093d-1c47-9f76-9ad44e791d7e endstream endobj 4 0 obj <>stream hÞ2W0P°±ÑwÎ/Í+Q0Ò÷ÎL)Ž64 )€ÉXýÊ‚Tý€ÄôÔb;;€œ¿ endstream endobj 5 0 obj <>stream hÞ\ÁªÂ0eo6‡šM¥Ä¢‰ÞÁ[LÖ¬lSÄ¿·>/Filter/FlateDecode/ID[]/Info 8 0 R/Length 45/Root 10 0 R/Size 9/Type/XRef/W[1 2 0]>>stream hÞbb```blûÁÄÀÄÉÄ tš‰c?ó11†¾eb``0[Û endstream endobj startxref 116 %%EOF