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The method contains all the GC)Tj (-)Tj (MS parameters needed for the analysis.)Tj ( )Tj 1.5 -1.66 Td (1.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (If you are not in the \223Instrument Control)Tj (\224 window, open it by choosing \223Instrument )Tj 0 -1.18 TD (Control\224 from the View menu.)Tj ( )Tj 0 -1.16 TD (Click on the Method menu, and select Load\205)Tj ( )Tj -1.5 -1.16 Td (2.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Select the method \223organic1.m\224.)Tj ( )Tj -1.5 -1.18 Td (3.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (After the method has been loaded, click on the button with the large gre\ en arrow to )Tj T* (start the analysis.)Tj ( )Tj -1.5 -1.16 Td (4.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Enter )Tj (your name in the \223Operator Name\224 box that appears)Tj ( )Tj -1.5 -1.18 Td (5.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Enter \223organic.d\224 in the data file name box. 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Not)Tj (e: )Tj -22.753 -1.18 Td (a Pasteur pipet contains about 2 mL of fluid when filled.)Tj ( )Tj -1.5 -1.16 Td (13.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Using another glass Pasteur pipet, add 1)Tj 6.96 0 0 6.96 311.6848 343.92 Tm ( )Tj 12 0 0 12 313.4348 344.88 Tm (\226)Tj 6.96 0 0 6.96 319.4348 343.92 Tm ( )Tj 12 0 0 12 321.1848 344.88 Tm (2 drops of your unknown sample to the )Tj -16.265 -1.16 Td (CH)Tj 6.96 0 0 6.96 144.0237 330 Tm (2)Tj 12 0 0 12 147.5237 330.96 Tm (Cl)Tj 6.96 0 0 6.96 158.8393 330 Tm (2)Tj 12 0 0 12 162.3394 330.96 Tm (, and mix.)Tj ( )Tj -4.528 -1.18 Td (14.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (Take a 10 \265L GC)Tj (-)Tj (MS syringe $make sure it has a blunt, cone shaped tip$ and pull )Tj T* (the plunger back )Tj (to the 1 \265L mark. Place the tip of the needle in your sample, and )Tj T* (withdraw the plunger to the 2 \265L mark. 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Choose \223Or)Tj (ganic1.m\224 from the list that )Tj T* (follows.)Tj ( )Tj -1.5 -1.2 Td (20.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (You can zoom)Tj (-)Tj (in on any peaks by holding down the left mouse button and drawing )Tj T* (a rectangle over the area you would like to zoom in on.)Tj 21.211 0 Td ( )Tj -22.711 -1.2 Td (21.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (You can zoom)Tj (-)Tj (out by double clicking the left mouse button anywhere on the )Tj T* (chromatog)Tj (ram.)Tj ( )Tj -1.5 -1.2 Td (22.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (To display the mass spectrum at any point in the chromatogram, double )Tj /T1_1 1 Tf 28.195 0 Td (right)Tj /T1_0 1 Tf ( )Tj (click )Tj -28.195 -1.2 Td (on a point in the chromatogram. The mass spectrum will be displayed belo\ w. 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Do not save the autointegration parameters to\ the Method. )Tj T* (You can view and print the integration report by selecting \223Integrati\ o)Tj 26.768 0 Td (n Results\205\224 or )Tj -26.768 -1.2 Td (\223Percent Report\224 from the same menu.)Tj ( )Tj -1.5 -1.2 Td (25.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (When you are finished analyzing your chromatogram, you can quit the )Tj /T1_1 1 Tf 27.668 0 Td (Data )Tj -27.668 -1.2 Td (Analysis)Tj /T1_0 1 Tf ( )Tj (program by selecting \223Exit\224 from the \223File\224 menu.)Tj ( )Tj /TT0 1 Tf 0.003 Tc -0.002 Tw 13.92 0 0 13.92 90 300 Tm [(F)1(i)1(ni)1(s)1(hi)1(ng)1( Up)]TJ 0 Tc 0 Tw 4.857 0 Td ( )Tj /T1_0 1 Tf 12 0 0 12 108 282 Tm (26.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (If you are finished with your unknown sample, throw the sol)Tj (vent away in the )Tj T* (Halogenated Organic Solvent waste bottle located in the Northern most fu\ me hood )Tj T* (in the Organic Chemistry lab.)Tj ( )Tj -1.5 -1.2 Td (27.)Tj /TT1 1 Tf ( )Tj /T1_0 1 Tf 1.5 0 Td (It is very important to clean the syringe out after every injection. You\ can do this by )Tj T* (putting a small amount of acetone in a be)Tj (aker, and drawing up 5)Tj 6.96 0 0 6.96 426.2341 223.44 Tm ( )Tj 12 0 0 12 427.9841 224.4 Tm (\226)Tj 6.96 0 0 6.96 433.9841 223.44 Tm ( )Tj 12 0 0 12 435.7341 224.4 Tm (10 \265L of acetone )Tj -25.811 -1.2 Td (into the syringe, followed by squirting it out into a kim)Tj 21.349 0 Td (-)Tj (wipe $where it will quickly )Tj -21.349 -1.2 Td (evaporate$. You should do this 5)Tj 6.96 0 0 6.96 276.5371 194.64 Tm ( )Tj 12 0 0 12 278.2871 195.6 Tm (\226)Tj 6.96 0 0 6.96 284.2871 194.64 Tm ( )Tj 12 0 0 12 286.0371 195.6 Tm (6 times to ensure the syringe is clean. Failure to do )Tj -13.336 -1.2 Td (this will result in carry over of your )Tj (sample into the next sample introduced into the )Tj T* (syringe. 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